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The side effect profile is dose-dependent, predictable, and in most cases manageable with proper preparation
Next, the cell lysates are centrifuged at 21000G for 20 minutes at 4 C and during this time, 30 uL of GFP-Trap beads (Bulldog Bio) per sample were washed 3 times in cold 1X RIPA buffer then aliquoted equally into microcentrifuge tubes for each sample
and Brower, D., "The PS2 Integrin Ligand Tiggrin is Required for Proper Muscle Function in Drosophila", Development 125: 1679-1687 (1998)
