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Resorufin formation at 37C was calculated by comparing the rate of increase in relative fluorescence to the fluorescence of known amounts of resorufin (excitation 562 mm, emission 586 nm)

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X-Ray and Magnetic Resonance Imaging (MRI) X-rays can be used to check for normal spinal alignment, bone spurs, narrowing and thinning of intervertebral disks that can affect the nerve cavity, spinal ligaments calcification, tumors, and more