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Vitamin B 1 deficiency causes overexpression of pro-inflammatory cytokines such as interleukin (IL)-1, IL-6, and tumor necrosis factor-alpha (TNF-) as well as increased expression of CD40 and CD40 ligand by microglial cells and astrocytes, which eventually leads to the death of neuron cells [18,19]

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The mouse fibroblast (3T3 L1) cell line showed a slightly higher cell density than the control group during early stages, but for cell viability, no significant difference was observed

[DOI] [PMC free article] [PubMed] [Google Scholar] 22.Wang M, Zhou X, Zhou S, Wang M, Jiang J, Wu W, Liu T, Xu W, Zhang J, Liu D, et al