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The expression was normalized using Nbubiquitin as a reference, and the 2CT method was used to calculate the level of gene expression (Yue et al., 2023)

Prognostic factors of craniopharyngioma with special reference to autocrine/paracrine signaling: underestimated implication of growth hormone receptor

The signal intensity of each band of target protein was measured and normalized to an internal loading control (either b-actin or vinculin) using Image Studio software (LI-COR Biosciences)

In Caco-2 intestinal epithelial cells, butyrate shows high responsiveness by activating the Nrf2 pathway
