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Cells should be induced at an early phase of the logarithmic growth curve for E
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Sarkhosh-Inanlou R, Molaparast M, Mohammadzadeh A, Shafiei-Irannejad V
Despite that UPR helps cells to cope with ER stress, prolonged ER stress induces apoptotic cell death where the accumulation of unfolded protein in ER results in increased expression activating transcription factor 4 (ATF-4) that in turn increases the transcription of the proapoptotic protein, C/EPB-homologous protein-10 (CHOP-10) (Li et al., 2014)
