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Oxidative modification of cysteine can occur, depending on the type and strength of the oxidant, as oxidation of the thiol to sulfenic, sulfinic, and sulfonic acids, formation of disulfides with GSH and other nucleophilic thiols, or Michael-type covalent adduction with small molecule electrophiles (3941)
After five passages, the surviving polyclonal population was sorted by fluorescence-activated cell sorting (FACS) based on expression of fluorescent markers, YPet (517/530 nm) and iRFP (690/713 nm) (Supplementary Fig

You would draw up to 2 units (about midway between the 2 and 3 marks)

During these sessions, only one lever was available at a time, with the available lever alternating between the right and left every second session