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We used glucagon and insulin to label cell and cell for co-staining, and found that the ratio of cell to cell in Model group increased observably, while the two groups treated with berberine improved this trend (Figure 2C, D)

[7] [9] The glucagon receptor component may contribute to increased energy expenditure and fat metabolism, though the human evidence for this effect is still evolving and should not be overstated

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For differential expression analysis, the R package limma (v.3.54.2) was used to identify differentially expressed proteins
